Molecular assay to fraud identification of meat products.
A novel template repairing-PCR technology based on miRNA-primed bypass synthesis at the abasic sites on the PCR template is developed as a sensitive and selective platform for miRNA detection. The assay is expected to show great promise for reverse transcription-free RNA PCR amplification and target-based co.
An Introduction to PCR Inhibitors By Joseph Bessetti Promega Corporation Given the wide range of PCR inhibitor-laden sample types and the options available for handling them, a multi-faceted approach is the best solution for amplification failure. DETECTION OF INHIBITORS Inhibition of multiplex STR amplifications can result in reduced product yield or complete failure. When inhibited samples.
This thesis describes two projects aimed at refining an established gene-profiling method, quantitative RT-PCR, so that it can be used to profile transcriptional-network states cross-sectionally within developing cell populations at single-cell resolution. Two advanced qRT-PCR protocols were developed to support these projects, one based on microfluidic “digital PCR,” the other based on.
Biology Dissertation Examples. The academic papers below were written by students to help you with your own studies. If you are looking for help with your work then we offer a comprehensive writing service provided by fully qualified academics in your field of study.
The polymerase chain reaction (PCR) is a relatively simple technique that amplifies a DNA template to produce specific DNA fragments in vitro. Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR requires only hours. While most biochemical analyses, including nucleic acid.
Polymerase chain reaction (PCR) is a way to make many copies of a sequence of DNA (this is sometimes called 'amplifying' the DNA). It is done in a lab, using an enzyme called DNA polymerase.It is called chain reaction because the result of one cycle is used immediately for the next cycle. This allows exponential growth to happen. PCR has many uses in a biological or biochemical setting.
RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to the two other commonly used techniques for quantifying mRNA levels, Northern blot analysis and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples. In fact, this technique is sensitive enough to.